Article
Deletions in epidermal keratins leading to alterations in filament organization in vivo and in intermediate filament assembly in vitro.
The Journal of cell biology - 1 Dec 1990
Coulombe P A, Chan Y M, Albers K, Fuchs E
Abstract excerpt
To investigate the sequences important for assembly of keratins into 10-nm filaments, we used a combined approach of (a) transfection of mutant keratin cDNAs into epithelial cells in vivo, and (b) in vitro assembly of mutant and wild-type keratins. Keratin K14 mutants missing the nonhelical carboxy- and amino-terminal domains not only integrated without perturbation into endogenous keratin filament networks in...
Topics
- Amino Acid Sequence
- Cells, Cultured
- Chromosome Deletion
- Epidermis
- Escherichia coli
- Humans
- Intermediate Filaments
- Keratins
- Macromolecular Substances
- Molecular Sequence Data
- Mutagenesis