Article
Identification of amino acids in HLA-DPw4b beta and -DR5 beta 1 chains that are involved in antibody binding epitopes using site-directed mutagenesis and DNA-mediated gene transfer.
Human immunology - 1 Feb 1990
Yu W Y, Watts R, Karr R W
Abstract excerpt
Based on comparisons of the amino acid sequences of the beta chains of HLA class II molecules that do or do not bind the I-LR1 monoclonal antibody, we predicted that glutamic acid 56 of I-LR1-positive DPw2, DPw3, and DPw4b beta chains and the analogous glutamic acid 58 of I-LR1-positive DR5 beta 1 chains are involved in the I-LR1 epitope. Site-directed mutagenesis of DPw4b beta and DR5 beta 1 cDNAs was used to...
Topics
- Amino Acid Sequence
- Antibodies, Monoclonal
- Binding Sites
- DNA
- Epitopes
- HLA-DP Antigens
- HLA-DP beta-Chains
- HLA-DR5 Antigen
- Humans
- Molecular Conformation
- Molecular Sequence Data