Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Evaluation of approaches to generation of tissue-specific knock-in mice.

The Journal of biological chemistry - 29 Sept 2006

Bayascas Jose R, Sakamoto Kei, Armit Laura, Arthur J Simon C, Alessi Dario R

Abstract excerpt

We explored three approaches to create tissue-specific knock-in mice by generating knock-in mice in which a substrate-docking site of the PDK1 protein kinase was ablated in Cre-expressing tissues in a way that prevented activation of one of its substrates, p70 ribosomal S6 kinase (S6K), but not another (protein kinase B (PKB)). Employing two of the approaches, termed the "heterozygous" and "minigene" methods, we...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to 3-Phosphoinositide-Dependent Protein Kinases
Share to Biological Transport

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genetic Techniques

Share to Genetic Vectors

Share to Glucose

Share to Insulin

Share to Proto-Oncogene Proteins c-akt

Share to Ribosomal Protein S6 Kinases, 70-kDa

Source

Identifiers

PMID: 16887794
DOI: 10.1074/jbc.M606789200

Genes

PDK1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Evaluation of approaches to generation of tissue-specific knock-in mice.PMID 16887794Read ↗

Detailed metabolic phenotyping of four tissue specific Cas9 transgenic mouse linesSemantic proximity Read ↗

Knockout Mice Challenge our Concepts of Glucose Homeostasis and the Pathogenesis of DiabetesSemantic proximity Read ↗

Knockout mouse models of insulin signaling: Relevance past and futureSemantic proximity Read ↗

Cre recombinase-dependent expression of a constitutively active mutant allele of the catalytic subunit of protein kinase A.Semantic proximity Read ↗

Cardiac-specific inducible and conditional gene targeting in mice.Semantic proximity Read ↗

The kick-in system: a novel rapid knock-in strategy.Semantic proximity Read ↗

Tissue-specific expression of Cas9 has no impact on whole-body metabolism in four transgenic mouse lines.Semantic proximity Read ↗

Generating double knockout mice to model genetic intervention for diabetic cardiomyopathy in humans.Semantic proximity Read ↗

A Muscle-Specific Insulin Receptor Knockout Exhibits Features of the Metabolic Syndrome of NIDDM without Altering Glucose ToleranceSemantic proximity Read ↗

Mutation of the PDK1 PH domain inhibits protein kinase B/Akt, leading to small size and insulin resistance.Semantic proximity Read ↗

Generation of a novel mouse model that recapitulates early and adult onset glycogenosis type IVSemantic proximity Read ↗

Mice expressing a human K(ATP) channel mutation have altered channel ATP sensitivity but no cardiac abnormalities.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.