Article
The kick-in system: a novel rapid knock-in strategy.
PloS one - 1 Jan 2014
Tomonoh Yuko, Deshimaru Masanobu, Araki Kimi, Miyazaki Yasuhiro, Arasaki Tomoko, Tanaka Yasuyoshi, Kitamura Haruna, Mori Fumiaki, Wakabayashi Koichi, Yamashita Sayaka, Saito Ryo, Itoh Masayuki, Uchida Taku, Yamada Junko, Migita Keisuke, Ueno Shinya, Kitaura Hiroki, Kakita Akiyoshi, Lossin Christoph, Takano Yukio, Hirose Shinichi
Abstract excerpt
Knock-in mouse models have contributed tremendously to our understanding of human disorders. However, generation of knock-in animals requires a significant investment of time and effort. We addressed this problem by developing a novel knock-in system that circumvents several traditional challenges by establishing stem cells with acceptor elements enveloping a particular genomic target. Once established, these...
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