Molecular diagnosis of alpha-thalassemia by combining real-time PCR with SYBR Green1 and dissociation curve analysis.

doi:10.1016/j.lab.2006.03.016

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Article

Molecular diagnosis of alpha-thalassemia by combining real-time PCR with SYBR Green1 and dissociation curve analysis.

Translational research : the journal of laboratory and clinical medicine - 1 Jul 2006

Liu Jingzhong, Yan Mei, Wang Zhangyong, Wang Lirong, Zhou Yan, Xiao Bai

Abstract excerpt

The aim of the study was to set up an automatic molecular diagnostic method for deletional alpha-thalassemia without gel electrophoresis and TaqMan probe. Four real-time polymerase chain reactions (PCRs) with SYBR Green1 and ABI7000 (SYBR-PCR) followed by dissociation curve (DC) analysis were used to detect the --(SEA), - alpha(3.7), -alpha(4.2), and non-deletion-type alleles (alpha alpha or alpha(T)alpha),...

Topics

Alleles
Automation
Benzothiazoles
Diamines
Fluorescent Dyes
Gene Deletion
Genotype
Humans
Nucleic Acid Denaturation
Organic Chemicals
Polymerase Chain Reaction
Quinolines
alpha-Thalassemia

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PMID: 16887492
DOI: 10.1016/j.lab.2006.03.016

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