Article
Detection of three common mutations causing β-thalassemia by using a closed-tube multiplex PCR.
Experimental and molecular pathology - 1 Oct 2018
Jia Xingyuan, Yao Limei, Zhou Yan, Han Han, Tang Ning, Wang Lirong, Li Ying
Abstract excerpt
Small oligonucleotides mutations are the large majority causes of β-thalassemia. Dual priming oligonucleotide PCR has been used to detect point mutations and thus could be applied to diagnose β-thalassemia. The goal of this study was to establish a simple, quick and cost-effective screening assay by using modified dual priming oligonucleotide PCR for three most common mutations of β-thalassemia [CD71-72 (+A), CD...
Topics
- Asian People
- China
- DNA Primers
- Genetic Testing
- Genotype
- Humans
- Multiplex Polymerase Chain Reaction
- Mutation
- Point Mutation
- beta-Thalassemia