Article
Rapid diagnosis of alpha(o)-thalassemia using the relative quantitative PCR and the dissociation curve analysis.
Clinical and laboratory haematology - 1 Dec 2003
Sangkitporn S K, Wangkahat K, Sangnoi A, Songkharm B, Charoenporn P, Sangkitporn S
Abstract excerpt
A method for diagnosis of alpha(o)-thalassemia was developed based on detection of accumulated PCR product using SYBR Green I, a double-stranded DNA binding dye, and a fluorescence-detecting thermocycler. Primers were designed to specifically amplify - -SEA and - -Thai deletions of alpha(o)-thalassemia. Albumin was selected as the reference gene. The comparative CT method was used to quantitate the target gene...
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