Article
A rapid detection for α-thalassemia by PCR combined with dissociation curve analysis.
Experimental and molecular pathology - 1 Oct 2011
Jia Xingyuan, Liu Jingzhong, Wang Lirong, Yao Limei, Tang Ning, Cai Ren, Xiao Bai
Abstract excerpt
Deletion mutations of 3.7 kb and 4.2 kb of α-globin gene are the most common causes of α-thalassemia (-α(3.7)/, -α(4.2)/). A simple, rapid assay by using a single-tube PCR to detect the two deletions has been needed. In this study, a pair of shared primers was designed for α2 and α1 gene but with length-different amplicons (159 bp and 409 bp). On the dissociation curve analysis profile after PCR, there shows two...
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