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Article

Improvement of lactic acid production in Saccharomyces cerevisiae by cell sorting for high intracellular pH.

Applied and environmental microbiology - 1 Aug 2006

Valli Minoska, Sauer Michael, Branduardi Paola, Borth Nicole, Porro Danilo, Mattanovich Diethard

Abstract excerpt

Yeast strains expressing heterologous L-lactate dehydrogenases can produce lactic acid. Although these microorganisms are tolerant of acidic environments, it is known that at low pH, lactic acid exerts a high level of stress on the cells. In the present study we analyzed intracellular pH (pHi) and viability by staining with cSNARF-4F and ethidium bromide, respectively, of two lactic-acid-producing strains of...

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Identifiers

PMID: 16885303
DOI: 10.1128/AEM.00683-06
PMCID: PMC1538745

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