Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A combination of flow cytometry and traditional screening using chemicals to isolate high glutathione-producing yeast mutants.

Bioscience, biotechnology, and biochemistry - 1 Jan 2012

Nishiuchi Hiroaki, Tabira Yukiko, Yamagishi Kazuo

Abstract excerpt

Traditional screening using chemicals or flow cytometry (FCM) alone is not sufficient to isolate the high glutathione (GSH)-producing yeast strains used in food production. Therefore, to improve screening efficiency, we investigated a combination of both methods. A mutated Saccharomyces cerevisiae strain was labeled with 5-chloromethylfluorescein diacetate and sorted by FCM according to emitted fluorescence...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Flow Cytometry
Share to Fluoresceins
Share to Fluorescent Dyes

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Glutathione

Share to High-Throughput Screening Assays

Share to Mutation

Share to Saccharomyces cerevisiae

Source

Identifiers

PMID: 22790927
DOI: 10.1271/bbb.110883

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A combination of flow cytometry and traditional screening using chemicals to isolate high glutathione-producing yeast mutants.PMID 22790927Read ↗

Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: color-based selection of ade1 or ade2 mutants.Semantic proximity Read ↗

Yeast as a tool to identify anti-aging compounds.Semantic proximity Read ↗

Preparation of a γ-glutamylcysteine-enriched yeast extract from a newly developed GSH2-deficient strain.Semantic proximity Read ↗

Discrimination of respiratory dysfunction in yeast mutants by confocal microscopy, image, and flow cytometry.Semantic proximity Read ↗

Hyper-activation of the target of rapamycin (Tor) kinase 1 decreases intracellular glutathione content in Saccharomyces cerevisiae as revealed by LC-MS/MS analysis.Semantic proximity Read ↗

A new method for isolation of S-adenosylmethionine (SAM)-accumulating yeast.Semantic proximity Read ↗

Systematic analysis of sporulation phenotypes in 624 non-lethal homozygous deletion strains of Saccharomyces cerevisiae.Semantic proximity Read ↗

A new approach for isolating cell wall mutants in Saccharomyces cerevisiae by screening for hypersensitivity to calcofluor white.Semantic proximity Read ↗

The construction and application of diploid sake yeast with a homozygous mutation in the FAS2 gene.Semantic proximity Read ↗

A positive screening for drugs that specifically inhibit the Ca2+-signaling activity on the basis of the growth promoting effect on a yeast mutant with a peculiar phenotype.Semantic proximity Read ↗

Isolation and characterization of baker's yeast capable of strongly activating a macrophage.Semantic proximity Read ↗

Development of Saccharomyces cerevisiae producing higher levels of sulfur dioxide and glutathione to improve beer flavor stability.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.