Article
Flow cytometric analysis of Saccharomyces cerevisiae autolytic mutants and protoplasts.
Yeast (Chichester, England) - 1 Jan 1992
de la Fuente J M, Alvarez A, Nombela C, Sanchez M
Abstract excerpt
Simple methods, based on the technique of flow cytometry, have been developed for the phenotypic characterization of yeast autolytic mutants and for the analysis of the formation and regeneration of the yeast protoplasts. The expression of lytic mutations determined uptake of the fluorescent dye propidium iodide, which could be carefully monitored by flow cytometry. Mixed populations of lysed and viable cells...
Topics
- Autolysis
- Cell Survival
- Culture Media
- Flow Cytometry
- Mutation
- Phenotype
- Propidium
- Protoplasts
- Saccharomyces cerevisiae