Phenotypic debrisoquine 4-hydroxylase activity among extensive metabolizers is unrelated to genotype as determined by the Xba-I restriction fragment length polymorphism.

Abstract excerpt

1. The major pathway for 4-hydroxylation of debrisoquine in man is polymorphic and under genetic control. More than 90% of subjects (extensive metabolizers, EMs) have active debrisoquine 4-hydroxylase (cytochrome P450IID6) while in the remainder (poor metabolizers, PMs), cytochrome P450IID6 activity is greatly impaired. 2. Within the EM group, cytochrome P450IID6-mediated metabolism of a range of substrates...

Topics

• Autoradiography
• Blotting, Southern
• Cytochrome P-450 CYP2D6
• Cytochrome P-450 Enzyme System
• DNA
• Encainide
• Genotype
• Humans
• Mixed Function Oxygenases
• Phenotype
• Polymorphism, Restriction Fragment Length