Article
Reporter assay systems for [URE3] detection and analysis.
Methods (San Diego, Calif.) - 1 May 2006
Brachmann Andreas, Toombs James A, Ross Eric D
Abstract excerpt
The Saccharomyces cerevisiae prion [URE3] is the infectious amyloid form of the Ure2p protein. [URE3] provides a useful model system for studying amyloid formation and stability in vivo. When grown in the presence of a good nitrogen source, [URE3] cells are able to take up ureidosuccinate, an intermediate in uracil biosynthesis, while cells lacking the [URE3] prion can not. This ability to take up ureidosuccinate...
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