Article
A new colour assay for [URE3] prion in a genetic background used to score for the [PSI⁺] prion.
Yeast (Chichester, England) - 1 Jul 2011
Hong Joo Y, Mathur Vidhu, Liebman Susan W
Abstract excerpt
Most scoring assays for yeast prions are dependent on specific genetic markers and constructs that differ for each prion. Here we describe a simple colour assay for the [URE3] prion that works in the 74D-964 strain frequently used to score the [PSI(+)] prion. Although this assay can only be used to score for [URE3] in the [psi(-)] version of the strain, it makes it easier to examine the effects of host mutations...
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