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Article

Rapid detection of rifampin resistance in Mycobacterium tuberculosis by Pyrosequencing technology.

Journal of clinical microbiology - 1 Jun 2006

Jureen Pontus, Engstrand Lars, Eriksson Solveig, Alderborn Anders, Krabbe Margareta, Hoffner Sven E

Abstract excerpt

We developed an assay for rapid detection of rifampin resistance in Mycobacterium tuberculosis based on Pyrosequencing technology, involving a technique for real-time sequencing. A 180-bp region of the rpoB gene was amplified in clinical isolates of both rifampin-resistant and -susceptible M. tuberculosis. The PCR products were subjected to Pyrosequencing analysis using four different sequencing primers in four...

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Identifiers

PMID: 16757581
DOI: 10.1128/JCM.02210-05
PMCID: PMC1489404

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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