Article
Development of a pyrosequencing approach for rapid screening of rifampin, isoniazid and ethambutol-resistant Mycobacterium tuberculosis.
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease - 1 Mar 2005
Zhao J-R, Bai Y-J, Wang Y, Zhang Q-H, Luo M, Yan X-J
Abstract excerpt
SETTING: The need to minimize the transmission of drug-resistant Mycobacterium tuberculosis requires rapid identification procedures. OBJECTIVE: To develop a pyrosequencing approach for rapid screening of rifampin, isoniazid and ethambutol-resistant M. tuberculosis based on characterization of resistance-associated hot mutations. DESIGN: Three pairs of PCR primers and three pyrosequencing sequencing primers for...
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