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Article

Monitoring protein interactions in the living cell through the fluorescence decays of the cyan fluorescent protein.

Chemphyschem : a European journal of chemical physics and physical chemistry - 17 Jul 2006

Grailhe Regis, Merola Fabienne, Ridard Jacqueline, Couvignou Stephen, Le Poupon Chantal, Changeux Jean-Pierre, Laguitton-Pasquier Helene

Abstract excerpt

Using fluorescence lifetime microspectroscopy and imaging techniques, we have studied the fluorescence of cyan fluorescent protein (CFP) transiently expressed in HEK-293 cells, in the presence or absence of its fluorescence resonance energy transfer (FRET) partner, yellow fluorescent protein (YFP). When the two proteins are attached through a 27-amino-acid linker, a 33 % average efficiency of intramolecular...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 16739159
DOI: 10.1002/cphc.200600057

Genes

CFP

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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