Article
Imaging FRET between spectrally similar GFP molecules in single cells.
Nature biotechnology - 1 Feb 2001
Harpur A G, Wouters F S, Bastiaens P I
Abstract excerpt
Fluorescence resonance energy transfer (FRET) detection in fusion constructs consisting of green fluorescent protein (GFP) variants linked by a sequence that changes conformation upon modification by enzymes or binding of ligands has enabled detection of physiological processes such as Ca(2+) ion release, and protease and kinase activity. Current FRET microscopy techniques are limited to the use of spectrally...
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