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Article

Cyan fluorescent protein carries a constitutive mutation that prevents its dimerization.

Biochemistry - 1 Feb 2011

Espagne Agathe, Erard Marie, Madiona Karine, Derrien Valérie, Jonasson Gabriella, Lévy Bernard, Pasquier Hélène, Melki Ronald, Mérola Fabienne

Abstract excerpt

The tendency of GFP-like fluorescent proteins to dimerize in vitro is a permanent concern as it may lead to artifacts in FRET imaging applications. However, we have found recently that CFP and YFP (the couple of GFP variants mostly used in FRET studies) show no trace of association in the cytosol of living cells up to millimolar concentrations. In this study, we investigated the oligomerization properties of...

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Source

Identifiers

PMID: 21175224
DOI: 10.1021/bi1015875

Genes

CFP

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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