Article
Direct molecular identification of the mouse pink-eyed unstable mutation by genome scanning.
Science (New York, N.Y.) - 26 Apr 1991
Brilliant M H, Gondo Y, Eicher E M
Abstract excerpt
DNA sequences associated with the mouse pink-eyed unstable mutation were identified in the absence of closely linked molecular markers and without prior knowledge of the encoded gene product. This was accomplished by "genome scanning," a technique in which high-resolution Southern blots of genomic DNAs were hybridized to a dispersed and moderately repetitive DNA sequence. In this assay, pink-eyed unstable DNA was...
Topics
- Alleles
- Animals
- Blotting, Southern
- DNA
- Eye Color
- Genes
- Homozygote
- Mice
- Mice, Inbred C57BL
- Mice, Mutant Strains
- Mutation
- Restriction Mapping