Article
Modulation of MthK potassium channel activity at the intracellular entrance to the pore.
The Journal of biological chemistry - 28 Jul 2006
Parfenova Lyubov V, Crane Brittany M, Rothberg Brad S
Abstract excerpt
We used a bacterial complementation screen with the LB2003 K(+) uptake-deficient strain of Escherichia coli to analyze residues that are critical to Methanobacterium thermoautotrophicum potassium channel (MthK) function. Channel expression and relative structural integrity of mutants were analyzed by SDS-PAGE and Western blot, and mechanisms underlying altered mutant channel function were analyzed using...
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