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Article

Opening the KcsA K+ channel: tryptophan scanning and complementation analysis lead to mutants with altered gating.

Biochemistry - 19 Nov 2002

Irizarry Stacey N, Kutluay Esin, Drews Gabriele, Hart Sarah J, Heginbotham Lise

Abstract excerpt

The properties of the KcsA channel were investigated using a combination of tryptophan scanning of the two transmembrane helices followed by random mutagenesis at targeted residues. The tryptophan mutants were subjected to two screens: oligomeric stability and ability to complement the K+ uptake deficiency of the TK2420 Escherichia coli strain. Oligomeric stability is affected primarily by mutations at sites that...

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PMID: 12427027
DOI: 10.1021/bi026393r

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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