Article
Functional complementation and genetic deletion studies of KirBac channels: activatory mutations highlight gating-sensitive domains.
The Journal of biological chemistry - 24 Dec 2010
Paynter Jennifer J, Andres-Enguix Isabelle, Fowler Philip W, Tottey Stephen, Cheng Wayland, Enkvetchakul Decha, Bavro Vassiliy N, Kusakabe Yoshio, Sansom Mark S P, Robinson Nigel J, Nichols Colin G, Tucker Stephen J
Abstract excerpt
The superfamily of prokaryotic inwardly rectifying (KirBac) potassium channels is homologous to mammalian Kir channels. However, relatively little is known about their regulation or about their physiological role in vivo. In this study, we have used random mutagenesis and genetic complementation in K(+)-auxotrophic Escherichia coli and Saccharomyces cerevisiae to identify activatory mutations in a range of...
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