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Article

Genetic selection of activatory mutations in KcsA.

Channels (Austin, Tex.) - 1 Jan 2000

Paynter Jennifer J, Sarkies Peter, Andres-Enguix Isabelle, Tucker Stephen J

Abstract excerpt

The KcsA potassium channel from Streptomyces lividans is one of the most actively studied ion channels. However, there are still unresolved issues about its gating mechanism in vivo because the channel is only activated by highly acidic intracellular pH, meaning that it will be mostly inactive in its host environment. In this study we have used a genetic complementation assay of K+-auxotrophic E. coli (TK2420)...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 18797191
DOI: 10.4161/chan.2.6.6874
PMCID: PMC2688072

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