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Article

Identifying off-target effects and hidden phenotypes of drugs in human cells.

Nature chemical biology - 1 Jun 2006

MacDonald Marnie L, Lamerdin Jane, Owens Stephen, Keon Brigitte H, Bilter Graham K, Shang Zhidi, Huang Zhengping, Yu Helen, Dias Jennifer, Minami Tomoe, Michnick Stephen W, Westwick John K

Abstract excerpt

We present a strategy for identifying off-target effects and hidden phenotypes of drugs by directly probing biochemical pathways that underlie therapeutic or toxic mechanisms in intact, living cells. High-content protein-fragment complementation assays (PCAs) were constructed with synthetic fragments of a mutant fluorescent protein ('Venus', EYFP or both), allowing us to measure spatial and temporal changes in...

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PMID: 16680159
DOI: 10.1038/nchembio790

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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