Article
Establishment and characterization of immortalized non-transplantable mouse mammary cell lines cloned from a MMTV-induced tumor cell line cultured for a long duration.
Nagoya journal of medical science - 1 Mar 1991
Takenaka T, Saga S, Miyaishi O, Imai M, Yokoi T, Lu J, Wajjwalku W, Kusakabe M, Taguchi O, Matsuyama M
Abstract excerpt
During the culturing of a mouse mammary tumor cell line, MuMT73, maintained in vitro for more than a decade, we found morphological heterogeneity in its cells; some showed contact inhibition in their growth, some formed domes and some grew criss cross and piled up. In trying to clone the cell line to isolate cells showing contact inhibition or dome formation, we were able to establish six clonal cell lines. These...
Topics
- Animals
- Antigens, Viral
- Clone Cells
- Cytoskeletal Proteins
- DNA, Neoplasm
- Extracellular Matrix Proteins
- Female
- Fibroblast Growth Factor 3
- Fibroblast Growth Factors
- Fluorescent Antibody Technique