Selective immortalization of a phenotypically distinct epithelial cell type by microinjection of SV40 DNA into cultured human milk cells.

doi:10.1002/ijc.2910450620

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Article

Selective immortalization of a phenotypically distinct epithelial cell type by microinjection of SV40 DNA into cultured human milk cells.

International journal of cancer - 15 Jun 1990

Bartek J, Bartkova J, Lalani E N, Brezina V, Taylor-Papadimitriou J

Abstract excerpt

An immortal cell line, MMSV-1, has been developed which exhibits many features of the common luminal epithelial cell of the human mammary gland. The cell line was developed by microinjection of SV40 DNA into individual cells in selected colonies in cultures of human milk epithelial cells. Immunohistochemical staining shows that the MMSV-1 cells express keratins 7, 8, 18 and 19 homogeneously in organized filaments...

Topics

Animals
Breast
Cell Line
Cell Survival
Cells, Cultured
Cytological Techniques
DNA, Viral
Epithelial Cells
Epithelium
Female
Humans

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Immunohistochemistry

Karyotyping

Mammary Neoplasms, Experimental

Mice

Mice, Nude

Microinjections

Milk, Human

Source

Open in PubMed

Identifiers

PMID: 2161799
DOI: 10.1002/ijc.2910450620

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2161799-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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