Article
Preparation of neocarzinostatin apoprotein mutants and the randomized library on the chromophore-binding cavity.
Biological & pharmaceutical bulletin - 1 May 2006
Tomioka Yoshihisa, Kisara Shigeki, Yoshizawa Saki, Ozawa Mika, Suzuki Naoto, Yamaguchi Hiroaki, Hishinuma Takanori, Mizugaki Michinao, Goto Junichi
Abstract excerpt
W39F, F52Y, S98G, S98A, and S98C mutants of the neocarzinostatin apoprotein (apo-NCS) were newly prepared and investigated their physicochemical properties. The circular dichroism (CD) spectra of F78W, F52Y, S98A, S98G, S98C were superimposable with that of wild type 1R49 protein although the minor spectral change seemed to be in the ellipticity of W39F. The results suggest that position 52, 78, and 98 involving...
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