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Article

Generation of a large library of point mutations in polyoma middle T antigen.

Nucleic acids research - 25 Dec 1991

Druker B J, Roberts T M

Abstract excerpt

Polyoma middle T antigen (MTAg) transforms cells by associating with and activating a variety of intracellular proteins, including src family members and a phosphatidylinositol-3 kinase. In order to assist in the study of the relative importance of the various associated biochemical activities for transformation by polyomavirus MTAg, a library of MTAg mutants was constructed. Chemically mutagenized MTAg DNA was...

Topics

Animals
Antigens, Polyomavirus Transforming
Cell Line
Cell Transformation, Viral
Cloning, Molecular
Electrophoresis
Gene Library
Genetic Vectors
Immunoblotting
Mice
Mice, Inbred BALB C

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Plasmids

Polyomavirus

Retroviridae

Source

Open in PubMed Open full text

Identifiers

PMID: 1662365
DOI: 10.1093/nar/19.24.6855
PMCID: PMC329320

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1662365-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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