Overexpression in Escherichia coli and purification of human fibroblast growth factor (FGF-2).

doi:10.1134/s000629790902014x

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Article

Overexpression in Escherichia coli and purification of human fibroblast growth factor (FGF-2).

Biochemistry. Biokhimiia - 1 Feb 2009

Gasparian M E, Elistratov P A, Drize N I, Nifontova I N, Dolgikh D A, Kirpichnikov M P

Abstract excerpt

Basic fibroblast growth factor (FGF-2) is a member of a large family of structurally related proteins that affect the growth, differentiation, migration, and survival of many cell types. The human FGF-2 gene (encoding residues 1-155) was synthesized by PCR from 20 oligonucleotides and cloned into plasmid pET-32a. A high expression level (1 g/liter) of a fused protein thioredoxin/FGF-2 was achieved in Escherichia...

Topics

Cloning, Molecular
Escherichia coli
Fibroblast Growth Factor 2
Humans
Mutation
Protein Multimerization
Recombinant Fusion Proteins
Solubility

Source

Open in PubMed

Identifiers

PMID: 19267679
DOI: 10.1134/s000629790902014x

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