Alterations in the ribosomal protein bL12 of E. coli affecting the initiation, elongation and termination of protein synthesis.

Abstract excerpt

In bacteria, ribosomal protein bL12 forms the prominent stalk structure on the ribosome and binds to multiple, distinct translational GTPase factors during the sequential steps of translation. Using a genetic selection in E. coli for altered readthrough of UGA stop codons, we have isolated seven different mutations affecting the C-terminal domain of the protein that forms the interaction surface with translation...

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