Heavy and Light Chain Variable Single Domains of an Anti-DNA Binding Antibody Hydrolyze Both Double- and Single-stranded DNAs without Sequence Specificity

Abstract excerpt

Anti-DNA antibodies (Abs) are of biomedical interest because they are associated with autoimmune diseases in human and mice. Previously we isolated an anti-DNA monoclonal Ab 3D8 from an autoimmune-prone MRL-lpr/lpr mouse. Here we have characterized DNA binding kinetics and hydrolyzing activities of the recombinant single chain variable fragment (scFv) and the single variable domains of heavy chain (VH) and light...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Chemical Synthesis and Analysis
• Share to DNA and Nucleic Acid Chemistry
• Share to Monoclonal and Polyclonal Antibodies Research