Article
Telomerase immortalization of human mammary epithelial cells derived from a BRCA2 mutation carrier.
Breast cancer research and treatment - 1 Sept 2006
Lewis Cheryl M, Herbert Brittney-Shea, Bu Dawei, Halloway Shane, Beck Adam, Shadeo Ashleen, Zhang Cindy, Ashfaq Raheela, Shay Jerry W, Euhus David M
Abstract excerpt
A novel human mammary epithelial cell line, HME348, was established from benign breast tissue from a 44-year-old germ-line BRCA2 mutation carrier with a history of stage 1 breast cancer. Mutation analysis showed that the patient had a known 6872del4 BRCA2 heterozygous mutation. The human mammary epithelial cells passaged in culture exhibited cellular replicative aging as evidenced by telomere shortening, lack of...
Topics
- Animals
- Breast
- Cell Culture Techniques
- Collagen
- Comet Assay
- Drug Combinations
- Epithelial Cells
- Genes, BRCA2
- Heterozygote
- Humans
- Laminin