Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10.

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10.

Cancer research - 15 Sept 1990

Soule H D, Maloney T M, Wolman S R, Peterson W D, Brenz R, McGrath C M, Russo J, Pauley R J, Jones R F, Brooks S C

Abstract excerpt

Two sublines of a breast epithelial cell culture, MCF-10, derived from human fibrocystic mammary tissue exhibit immortality after extended cultivation in low calcium concentrations (0.03-0.06 mM) and floating transfers in low calcium (MCF-10F), or by trypsin-Versene passages in the customary (nor...

Topics

Animals
Breast Neoplasms
Cell Count
Chromosome Aberrations
Female
Genotype
Humans
Mice
Proto-Oncogene Proteins
Proto-Oncogenes
Receptor, ErbB-2
Simian virus 40
Tumor Cells, Cultured

Source

Open in PubMed

Identifiers

PMID: 1975513

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1975513-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics