Article
Novobiocin-dependent topA deletion mutants of Escherichia coli.
Journal of bacteriology - 1 Sept 1991
Hammond G G, Cassidy P J, Overbye K M
Abstract excerpt
Previous reports of the transduction of topA deletions in Escherichia coli suggested that delta top A transductants grow normally only if they acquire spontaneous mutations that compensate for the topoisomerase I defect. We show that P1-mediated transduction of delta topA in the presence of sublethal concentrations of novobiocin, an inhibitor of the DNA gyrase B subunit, yields uncompensated Top- isolates which...
Topics
- Chromosome Deletion
- DNA Topoisomerases, Type I
- Drug Resistance, Microbial
- Escherichia coli
- Genes, Bacterial
- Mutation
- Novobiocin
- Transduction, Genetic