Genetic selection of regulatory mutants of mammalian adenylyl cyclases.

doi:10.1016/s0076-6879(02)45020-3

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Article

Genetic selection of regulatory mutants of mammalian adenylyl cyclases.

Methods in enzymology - 1 Jan 2002

Clapp Peter, Capper Austin B, Taussig Ronald

Abstract excerpt

Initial steps in the identification of the Gs alpha-binding site present in mammalian adenylyl cyclases can be achieved with the use of the yeast genetic system described. It must be stressed that this system serves as a means to identify mutants that are candidates; biochemical analysis of these mutants is a next and necessary step in the confirmation of these phenotypes. The system described can be readily...

Topics

Adenylyl Cyclases
Animals
Dogs
GTP-Binding Protein alpha Subunits, Gs
Gene Library
Genetic Techniques
In Vitro Techniques
Isoenzymes
Mutagenesis
Mutation
Recombinant Proteins
Saccharomyces cerevisiae
Transformation, Genetic

Source

Open in PubMed

Identifiers

PMID: 11665608
DOI: 10.1016/s0076-6879(02)45020-3

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