Article
Genetic selection of regulatory mutants of mammalian adenylyl cyclases.
Methods in enzymology - 1 Jan 2002
Clapp Peter, Capper Austin B, Taussig Ronald
Abstract excerpt
Initial steps in the identification of the Gs alpha-binding site present in mammalian adenylyl cyclases can be achieved with the use of the yeast genetic system described. It must be stressed that this system serves as a means to identify mutants that are candidates; biochemical analysis of these mutants is a next and necessary step in the confirmation of these phenotypes. The system described can be readily...
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