Article
Sequential N- to C-terminal SNARE complex assembly drives priming and fusion of secretory vesicles.
The EMBO journal - 8 Mar 2006
Sørensen Jakob B, Wiederhold Katrin, Müller Emil M, Milosevic Ira, Nagy Gábor, de Groot Bert L, Grubmüller Helmut, Fasshauer Dirk
Abstract excerpt
During exocytosis a four-helical coiled coil is formed between the three SNARE proteins syntaxin, synaptobrevin and SNAP-25, bridging vesicle and plasma membrane. We have investigated the assembly pathway of this complex by interfering with the stability of the hydrophobic interaction layers holding the complex together. Mutations in the C-terminal end affected fusion triggering in vivo and led to two-step...
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