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Article

Prospective isolation of murine hematopoietic stem cells by expression of an Abcg2/GFP allele.

Stem cells (Dayton, Ohio) - 1 Jun 2006

Tadjali Mehrdad, Zhou Sheng, Rehg Jerold, Sorrentino Brian P

Abstract excerpt

Stem cells from a variety of tissues can be identified by a side population (SP) phenotype based on Hoechst 33342 dye efflux. The Abcg2 transporter is expressed in hematopoietic stem cells (HSCs) and confers this dye efflux activity. To further explore the relationship among Abcg2 expression, the SP phenotype, and HSC activity, we have generated mice in which a green fluorescent protein (GFP) reporter gene was...

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PMID: 16484343
DOI: 10.1634/stemcells.2005-0562

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