Article
Zebrafish kidney marrow contains ABCG2-dependent side population cells exhibiting hematopoietic stem cell properties.
Differentiation; research in biological diversity - 1 Mar 2007
Tsinkalovsky Oleg, Vik-Mo Audun Osland, Ferreira Sara, Laerum Ole Didrik, Fjose Anders
Abstract excerpt
Zebrafish (Danio rerio) has emerged as a powerful genetic model for the study of vertebrate hematopoiesis. However, methods for detection and isolation of hematopoietic stem cells (HSCs) have not yet been reported. In mammals, the combination of Hoechst 33342 staining with flow cytometry can be used for separation of a bone marrow side population (SP), which is highly enriched for HSCs. We applied a similar...
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