Article
Detection and enrichment of hematopoietic stem cells by side population phenotype.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2004
Eaker Shannon S, Hawley Teresa S, Ramezani Ali, Hawley Robert G
Abstract excerpt
A flow cytometric procedure has recently been described to isolate hematopoietic stem cells from mouse bone marrow based on the efflux properties of the vital dye Hoechst 33342. The assay defines a subset of cells-termed the "side population" (SP)-by simultaneously measuring fluorescence of the d...
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