Article
Splice site requirement for the efficient accumulation of polyoma virus late mRNAs.
Nucleic acids research - 11 Jun 1991
Barrett N L, Carmichael G G, Luo Y
Abstract excerpt
Polyoma virus late nuclear primary transcripts are giant and heterogeneous, containing tandem repeats of the late strand of the circular viral genome. Late pre-mRNA processing involves the splicing of noncoding 'leader' exons to each other (removing genome-length introns), with the joining of the last leader to a coding 'body' exon. We have constructed a number of mutants blocked only in leader-leader splicing,...
Topics
- Base Sequence
- Cell Line
- Genes, Viral
- Introns
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Polyomavirus
- RNA Splicing
- RNA, Messenger
- RNA, Viral
- Transcription, Genetic