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Article

Thermal stabilization of Bacillus subtilis family-11 xylanase by directed evolution.

The Journal of biological chemistry - 14 Apr 2006

Miyazaki Kentaro, Takenouchi Misa, Kondo Hidemasa, Noro Natsuko, Suzuki Mamoru, Tsuda Sakae

Abstract excerpt

We used directed evolution to enhance the thermostability of glycosyl hydrolase family-11 xylanase from Bacillus subtilis. By combining random point mutagenesis, saturation mutagenesis, and DNA shuffling, a thermostable variant, Xyl(st), was identified which contained three amino acid substitutions: Q7H, N8F, and S179C. The half-inactivation temperature (the midpoint of the melting curves) for the Xyl(st) variant...

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PMID: 16467302
DOI: 10.1074/jbc.M511948200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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