Article
High-temperature behavior of hyperthermostable Thermotoga maritima xylanase XYN10B after designed and evolved mutations.
Applied microbiology and biotechnology - 1 Mar 2022
Wang Yawei, Wang Jing, Zhang Zhongqiang, Yang Jiangke, Turunen Ossi, Xiong Hairong
Abstract excerpt
A hyperthermostable xylanase XYN10B from Thermotoga maritima (PDB code 1VBR, GenBank accession number KR078269) was subjected to site-directed and error-prone PCR mutagenesis. From the selected five mutants, the two site-directed mutants (F806H and F806V) showed a 3.3-3.5-fold improved enzyme half-life at 100 °C. The mutant XYNA generated by error-prone PCR showed slightly improved stability at 100 °C and a lower...
Topics
- Endo-1,4-beta Xylanases
- Enzyme Stability
- Models, Molecular
- Mutation
- Temperature
- Thermotoga maritima