Article
Improved thermostability of an acidic xylanase from Aspergillus sulphureus by combined disulphide bridge introduction and proline residue substitution.
Scientific reports - 8 May 2017
Yang Wenhan, Yang Yongzhi, Zhang Lingdi, Xu Hang, Guo Xiaojing, Yang Xu, Dong Bing, Cao Yunhe
Abstract excerpt
As a feed additive, xylanase has been widely applied in the feed of monogastric animals, which contains multiple plant polysaccharides. However, during feed manufacture, the high pelleting temperatures challenge wild-type xylanases. The aim of this study was to improve the thermostability of Aspergillus sulphureus acidic xylanase. According to the predicted protein structure, a series of disulphide bridges and...
Topics
- Amino Acid Substitution
- Aspergillus
- Disulfides
- Fermentation
- Gene Expression
- Hydrogen-Ion Concentration
- Models, Molecular
- Mutation
- Proline
- Protein Conformation