Article
Improving the Thermostability of Xylanase A from Bacillus subtilis by Combining Bioinformatics and Electrostatic Interactions Optimization.
The journal of physical chemistry. B - 6 May 2021
Ngo Khoa, Bruno da Silva Fernando, Leite Vitor B P, Contessoto Vinícius G, Onuchic José N
Abstract excerpt
The rational improvement of the enzyme catalytic activity is one of the most significant challenges in biotechnology. Most conventional strategies used to engineer enzymes involve selecting mutations to increase their thermostability. Determining good criteria for choosing these substitutions continues to be a challenge. In this work, we combine bioinformatics, electrostatic analysis, and molecular dynamics to...
Topics
- Bacillus subtilis
- Bacterial Proteins
- Computational Biology
- Endo-1,4-beta Xylanases
- Enzyme Stability
- Mutation
- Static Electricity