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Article

Cooperative hydrogen bond interactions in the streptavidin-biotin system.

Protein science : a publication of the Protein Society - 1 Mar 2006

Hyre David E, Le Trong Isolde, Merritt Ethan A, Eccleston John F, Green N Michael, Stenkamp Ronald E, Stayton Patrick S

Abstract excerpt

The thermodynamic and structural cooperativity between the Ser45- and D128-biotin hydrogen bonds was measured by calorimetric and X-ray crystallographic studies of the S45A/D128A double mutant of streptavidin. The double mutant exhibits a binding affinity approximately 2x10(7) times lower than that of wild-type streptavidin at 25 degrees C. The corresponding reduction in binding free energy (DeltaDeltaG) of 10.1...

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Identifiers

PMID: 16452627
DOI: 10.1110/ps.051970306
PMCID: PMC2249767

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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