Article
A streptavidin mutant with altered ligand-binding specificity.
Proceedings of the National Academy of Sciences of the United States of America - 10 Nov 1998
Reznik G O, Vajda S, Sano T, Cantor C R
Abstract excerpt
The biotin-binding site of streptavidin was modified to alter its ligand-binding specificity. In natural streptavidin, the side chains of N23 and S27 make two of the three hydrogen bonds with the ureido oxygen of biotin. These two residues were mutated to severely weaken biotin binding while atte...
Topics
- Binding Sites
- Biotin
- Drug Design
- Escherichia coli
- Ligands
- Mutation
- Protein Conformation
- Streptavidin