Structural studies of binding site tryptophan mutants in the high-affinity streptavidin-biotin complex.

doi:10.1006/jmbi.1998.1735

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Article

Structural studies of binding site tryptophan mutants in the high-affinity streptavidin-biotin complex.

Journal of molecular biology - 29 May 1998

Freitag S, Le Trong I, Chilkoti A, Klumb L A, Stayton P S, Stenkamp R E

Abstract excerpt

Previous thermodynamic and computational studies have pointed to the important energetic role of aromatic contacts in generating the exceptional binding free energy of streptavidin-biotin association. We report here the crystallographic characterization of single site tryptophan mutants in invest...

Topics

Binding Sites
Biotin
Crystallography, X-Ray
Models, Molecular
Mutation
Protein Conformation
Streptavidin
Tryptophan

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