Article
A structural snapshot of an intermediate on the streptavidin-biotin dissociation pathway.
Proceedings of the National Academy of Sciences of the United States of America - 20 Jul 1999
Freitag S, Chu V, Penzotti J E, Klumb L A, To R, Hyre D, Le Trong I, Lybrand T P, Stenkamp R E, Stayton P S
Abstract excerpt
It is currently unclear whether small molecules dissociate from a protein binding site along a defined pathway or through a collection of dissociation pathways. We report herein a joint crystallographic, computational, and biophysical study that suggests the Asp-128 --> Ala (D128A) streptavidin mutant closely mimics an intermediate on a well-defined dissociation pathway. Asp-128 is hydrogen bonded to a ureido...
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