Article
Analyzing the genetic characteristics of a tryptophan-overproducing Escherichia coli.
Bioprocess and biosystems engineering - 1 Aug 2021
Ding Dongqin, Bai Danyang, Li Jinlong, Mao Zhitao, Zhu Yaru, Liu Pi, Lin Jianping, Ma Hongwu, Zhang Dawei
Abstract excerpt
L-tryptophan (L-trp) production in Escherichia coli has been developed by employing random mutagenesis and selection for a long time, but this approach produces an unclear genetic background. Here, we generated the L-trp overproducer TPD5 by combining an intracellular L-trp biosensor and fluorescence-activated cell sorting (FACS) in E. coli, and succeeded in elucidating the genetic basis for L-trp overproduction....
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